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doi: 10.1016/j.pcl.2015.08.008 25 BernasconiSLevyCCohenRGiardinoIPettoello-MantovaniMSomekhE

Functional analysis of the glycogen binding subunit CG9238/Gbs-70E of protein phosphatase 1 in Drosophila melanogaster

Immunofluorescence and fluorescence complementation For immunofluorescence of cultured cells, the cells were fixed with 4% paraformaldehyde (PFA) for 15 min at room temperature, permeabilized with 0.1% TritonX-100 diluted in PBS at room temperature for 5 min, blocked with 10% FBS diluted with PBS for 1 h and primary antibody incubation for 1 h

For comparison of the CMap data to our in vitro studies of the effects of GHK in primary lung fibroblasts, raw data for GHK-treated and control samples were downloaded from the CMap website and normalized using MAS5.0 with the Affymetrix CDF