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Furthermore, WB analysis was conducted on extracts from the aortas of aging mice, showing consistent results with in vitro experiments: increased expression of p-P53 and decreased expression of total P53 after FOXO4-DRI treatment

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However, after EGCG treatment, embryo developmental competence was improved, as indicated by the increased ICM and total cell number, in association with the reductions in the numbers of damaged and apoptotic cells in blastocysts developed from H 2 O 2 /EGCG-treated zygotes