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The pellet containing the proteins was reconstituted with 100 mM Tris (pH 8.5) and 2% sodium deoxycholate and alkylated by adding 5 mM TCEP and 30 mM chloroacetamide at 56 C for 10 min

3a) and incubated GST-tagged (P)-SF3B1 (1-463) with his-tagged wild-type or FHA-deletion mutant (del FHA) SNIP1 proteins expressed in E

It is believed that the enzyme l-GalLDH may influence the respiratory process (Millar et al., 2003), bypassing the phosphorylating respiratory Complexes I and III ( Figure 4B )