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The process of macroautophagy (herein referred to as autophagy) can be divided into 5 stages: initiation, elongation, maturation, fusion and degradation [8]

The rat s liver tissues were used to perform pathological examinations, second-generation sequencing (RNA-seq), and proteomics analysis

Metformin treatment (100 mg/kg) showed the most significant protective effect, with a marked reduction in lipid accumulation, improved cellular integrity, and minimal evidence of necrosis or other pathological features ( E )