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To this end we investigated the consequences of T3 treatment in an experimental model of ischemic stroke in mice Methods: Stroke was induced in C57BL/6 WT mice by transient middle cerebral artery occlusion (tMCAO)

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The amount of cysteine or cysteamine released was determined by adding 1 mM BrP forming cyclic sulfur products (lanthionine ketimine and aminoethylcysteine ketimine) that absorb at 296 nm ( = 6200 M 1 cm 1 ), while after adding NaOH (20 mM final concentration) to the solution the homocysteine released was determined by the reaction with 1 mM BrP

RPMI 1640 medium was from Biochrom