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The Metabolome vs Other MoA Evidence Layers Mechanism-of-action research increasingly draws on multiple molecular layers proteomics, transcriptomics, and metabolomics each record the consequences of drug action at a different level of biological organisation

The upregulation of GPX6 expression found in these two studies suggests that its upregulation may have some antioxidant effects

A crucial step will be to perform a rigous retrospective validation after harmonized preprocessing (including consistent expression units and rigorous batch correction) to ensure the technical reliability of the analysis

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