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J Crohns Colitis 2024;18:16151621.ArticlePubMedPDF 8

Arginine is reduced to citrulline in a five-electron oxidative reaction by nitric oxide synthases (NOSs) and this reaction gives rise to NO

This activity relies to the binding of a heme, which is stabilized by hydrogen bonds when a glutathione is present in the active site (Yamada and Takusagawa, 2007