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10.1006/taap.2000.9083 Toxicol Appl Pharmacol

Fragment 176-191 is a peptide that is a molecular fragment of Human Growth Hormone (HGH) consisting of 16 amino acids attached to the C-terminus of positions 176 and 191

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For fermentative production, the cells were grown in SM1 minimal medium (1 g/L Trypton, 0.5 g/L Yeast extract, 100 M potassium phosphate buffer (pH 7.0), 75.7 M (NH 4 ) 2 SO 4 , 1.7 M NaCl, 1.0 M MgSO 4 , 0.1 M CaCl 2 , 20 mg/L ammonium ferric citrate (AFC), 0.6 M Na 2 MoO 4 , 40.4 M H 3 BO 3 , 2.9 M CoCl 2 , 1 M CuSO 4 , 8.1 M MnCl 2 , 3.04 mM Met) supplemented with 3% glucose