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Interestingly, using the 3- 2 H-glucose tracing approach 66 we found that, in unstimulated neutrophils, the active hydride of NADPH barely labelled from 3- 2 H-glucose but the fraction of 2 H-labelled NADPH increased drastically following PMA stimulation (Extended Data Fig

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Even if BME is difficult to hydrolyze to biotin within cells, it is expected to improve the temporal control of the RUSH system, because the carboxyl group of biotin itself is not involved in its binding to streptavidin 22

This forces the cell into early cellular death