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Briefly, cells were seeded in 6-well plates at a density of 2.5 10 5 cells/well and incubated at 37C overnight

We adhere steadfastly to all regulatory guidelines without compromise

Applying the biomarkers in initial single and multiple ascending dose clinical studies could help inform planned dose escalations or continued dosing schedules

When the normality assumption was uncertain, as was determined for plasma cytokines, non-parametric KruskalWallis test and MannWhitney U test were used