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2.3 Bone marrow-derived macrophage (BMDM) isolation and culture BMDMs were derived from the bone marrow, which was isolated from male C57Bl/6 mice and cultured in DMEM (HyClone) supplemented with 1% penicillin/streptomycin (Invitrogen), 10 ng/mL M-CSF (Peprotech) and 10% FBS (Invitrogen)

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Betaine is utilised in most tissues as an osmoproctectant in addition to its primary role as a methyl group donor in liver metabolism (reviewed by Zhao et al, 2018)

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