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Xu P, Han N, Kang T, Zhan S, Lee KS, Jin BR, et al

coli containing pET-28b(+) based plasmids (pANT13-16, pANT18, pANT20-21, pANT35, pANT95, pJK1-3, pJK7-9 or site saturation variants of pANT35) were inoculated from an overnight preculture at a ratio of 1:100 in 96-deep-well plates equipped with a CR1296 sandwich cover (EnzyScreen) in 0.5 ml TB medium containing 50 g ml 1 kanamycin sulfate per well and incubated at 37 C, 300 rpm using a 5 cm shaking diameter for 2.5 h

We performed additional quality control to remove proteins with a high coefficient of variation or a low correlation between the two different versions of the SomaScan assay present across our cohorts, leaving us with 4,778 proteins (856 organ enriched, 17.9%) which were used for downstream analysis (Supplementary Fig

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