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Equivalent proteins were then separated by 10% Tris-Tricine SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membranes

J Gastroenterol Hepatol 27(8):12661280 Spehlmann ME, Begun AZ, Burghardt J, Lepage P, Raedler A, Schreiber S (2008) Epidemiology of inflammatory bowel disease in a German twin cohort: results of a nationwide study