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Site-directed mutagenesis was performed using a standard two-step PCR, followed by purification using StarPrep Gel Extraction Kit (D205-04, GenStar, Beijing) and verified by DNA sequencing (Sangon Biotech, Shanghai)

Glutathione plays a central role in detoxification, cellular protection, and managing oxidative stress systems that often require extra support when methylation is reduced

Redox signaling: an evolution from free radicals to aging

The activation phase is triggered by a variety of stimuli such as pore-forming bacterial toxins, extracellular ATP, or particulate matter like uric acid crystals, silica, and asbestos