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Medicine, 104(5), e41399.

The application of microneedles in the field of natural medicines and pathological scars has been widely studied since 2021, with 11 relevant articles included in this study

R.MehrganM

Representative tissue specimens were collected, dehydrated and embedded in parafn blocks, sectioned at 4 m, and stained with hematoxylin-eosin according to the following automated Sakura Tissue-Tek DRS 2000 Slide Stainer protocol ( Coverslipping/H-E-Kit accessed on 19 October 2021): rehydration in distilled water, stain- Biomedicines 2022,10, 265 6 of 38 ing with hematoxylin, washing in running tap water, differentiation with 70% alcohol, staining with eosin, dehydration, clearing, and mounting