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Bound proteins were eluted with glutathione buffer [10 mM reduced glutathione (Wako), 50 mM Tris pH 8.0, 500 mM NaCl, 10 mM -mercaptoethanol, and 10% glycerol]

In the brain, SELENOP is primarily expressed in astrocytes, but neurons have also been identified as a source of endogenous SELENOP through the entire brain (Steinbrenner et al., 2006

In this phase, the liver attaches a glutathione molecule to a toxin, making that toxin water-soluble so it can be safely flushed out of the body through the kidneys or gut

the predicted binding mode of 1j indicates the C5-amino substituent forms hydrogen bonding interaction with the carboxylic backbone of the Ser201 residue and a hydrophobic bonding with the Ser213 residue unlike the NCA amide group that is in hydrogen bonding distance from with the NNMT Ser213 residue