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Flies were then washed (twice for 1 min each) with 0.15 M sodium cacodylate buffer pH 7.4 containing 2 mM CaCl 2 on ice and were reacted with 2.5 mM Ce-DAB2 solution in cacodylate buffer 36 for 75 min to produce the desired brown intensity color from the precipitate

2018 Jan 3;97(1):1-2

However, the number of ongoing studies indicates the therapeutic potential of the KD, and implementation of this results of these trials will enable the verification of the hypothesis

During the time-restricted eating (TRE) (or time restricted feeding -TRF- in animal models) daily eating hours are reduced (Liu et al., 2022), allowing food intake ad libitum in windows ranging from 4 up to 10 h (1420 h of fasting per day) (Lowe et al., 2020