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Pechlaner R, Tsimikas S, Yin X, Willeit P, Baig F, Santer P, Oberhollenzer F, Egger G, Witztum JL, Alexander VJ, Willeit J, Kiechl S, Mayr M

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Immunofluorescence Staining and Confocal Imaging Enteroid monolayers were fixed for 40 min in 4% paraformaldehyde (Electron Microscopy Sciences, United States), washed with PBS for 10 min, permeabilized and blocked for 1 h with PBS containing 15% fetal bovine serum, 2% BSA, and 0.1% saponin, all at room temperature (RT)

Detection of peroxynitrite accumulation in Arabidopsis thaliana during the hypersensitive defense response