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2.3 Cell culture and treatment Keratinocytes and skin fibroblasts were cultured separately to assess the effects of kimchi extract on skin cells, as previously described [18] with some modifications

Monica B

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In the absence of oxygen, there is little mitochondrial NAD + available for the pyruvate dehydrogenase complex, thus no acetyl-CoA will be formed, pyruvate carboxylase will not get activated, there will be no citrate, there will be no citric acid cycle, ATP levels will be low, thus phosphofructokinase-1 will remain uninhibited (because of no citrate and low [ATP] accompanied by high [ADP])