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Finally, the membranes were washed with PBST three times, and a ChemiDoc XRS System (Bio-Rad, CA, USA) was used to detect the expression of proteins with an enhanced chemiluminescence (ECL) kit (NCM Biotech, Suzhou, China)

PMID: 58 MaX.ZhangB.XiangX.LiW.LiJ.LiY.et al

NAFL is defined as hepatic steatosis without substantial hepatocyte injury, or minor lobular inflammation is present, while NASH is characterized by hepatocyte damage in addition to steatosis (54)

The endoplasmic reticulum (ER) is responsible for lipid biosynthesis in cells, arising as the main source of essential components of membrane bilayers such as phospholipids and cholesterol, which are distributed to different intracellular compartments, including mitochondria