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The final conjugate was buffer-exchanged/purified with either PBS pH 7.4 buffer or with histidine sucrose buffer (20 mM histidine buffer pH 6.0, 4% (w/v) sucrose + 75 mM NaCI) by four rounds of dilution/centrifugation using Amicon 30K centrifugal filters device and were sterile-filtered (0.20pm PES filter)

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