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3 were acquired using Titan Krios cryo-TEM (ThermoFisher Scientific) equipped with a Gatan Bioquantum energy filter and a K3 Summit direct detector at an acceleration voltage of 300 kV using SerialEM 66 at a magnification of 19,500, at binning 2, corresponding to a pixel size of 0.9 nm at a defocus of 30 m

In addition to its basic antioxidant function, FSP1 can also induce inhibition of ferroptosis from another aspectmembrane repair action via endosomal sorting complex required for transport -III (Zhang et al., 2024a), yet these results need deeper research

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Edu staining experiment Place KGN cells on a coverslip in a 24-well plate and culture until confluent