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Many of these trials recruited terminal or end-stage patients, for whom any sort of treatment is unlikely to have an effect, and they often recruited cohorts with mixed cancers, which may respond differentially to IVC depending on the underlying mechanisms involved

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ThermoFisher Scientific #29202) were briefly centrifuged (1000 g ) and washed once with 8 mL 50 mM HEPES buffer (pH 7.5) and two times with 8 mL of the same buffer supplemented with 1 mM TCEP