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Thus, intraperitoneal injection of NMN (500 mg/kg) into 20-month-old BESTO mice led to an enhancement in GSIS and improvement in glucose tolerance in the aged BESTO females instead of males

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To interrogate whether melatonin could restrain ILC2 effector function we employed an adoptive transfer model, whereby activated ILC2s were isolated from Rag2 -/- mice, cultured for 24h in the presence or absence of melatonin, and then adoptively transferred the ILC2s into Rag2 -/- GC -/- mice utilizing established protocol as described by our group previously (48, 49) ( Figure 4A )

Archives neurology 66 (2), 216225