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When lysosomes were disrupted by LLOME treatment, SCF FBXO27 was rapidly recruited to the broken lysosomes and ubiquitinated LAMP-2 to activate lysophagy 85

Tang C, Border JJ, Zhang H, Gregory A, Bai S, Fang X, Liu Y, Wang S, Hwang SH, Gao W et al

297 These results suggest that baicalein may prevent ferroptosis-associated tissue damage

The major breakthrough was the discovery of a biotinylated polyclonal goat anti-EPO antibody (BAF959, RnD Systems), that used in combination with a proprietary streptavidin-HRP conjugate, allowed this technique to be sensitive enough to be used for initial testing procedure