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Impact of nutritional and environmental factors on inflammation, oxidative stress, and the microbiome

But would that be us

To select for successfully transduced cells, puromycin was added to the medium at a concentration of 1 g/mL, and the selection was continued for 14 days until non-transduced cells were eliminated

This was evidenced by the downregulation of cytoplasmic mtDNA-related cGAS-STING and NLRP3 inflammasome signaling pathways, as well as reduced expression of proinflammatory factors IL6, IL1, and TNF- in Tibetan M9a+sh EPAS1 cells