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The membrane was blocked for 1 h in 5% low-fat milk or BSA prepared in Tris-buffered saline containing 0.1% Tween-20 (TBST), and then incubated for over-night with the indicated primary antibodies followed by incubation with HRP-conjugated goat anti-rabbit or goat anti-mouse IgG (1: 10,000

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The whole South American Plate, including the Atacama Desert and its associated continental slope, have remained under stable latitudinal and hyper-arid conditions for the past 200 million years 2