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Due to various problems during sectioning and staining, 7 samples were excluded after the staining procedure, distributed in the following groups: 2 for collagen 1 (RL 1 week, FL 20 weeks), 3 for collagen 3 (ML 1 week, RL 1 week, RL 2 weeks), and 2 for elastin (RL 1 week, FL 3 weeks)

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Several recombinant nitrilases have been successfully cloned and characterized to produce nicotinic acid with improved specific activity by several-fold ( Pseudomonas putida CGMCC3830 showed 84.0 U/mg specific activity towards 27.9 mM 3-cyanopyridine ( TABLE 3 Recombinant strains reported for the production of nicotinic acid