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Adv In Microb Physiol 49:276 Qiu Z, Deng Z, Tan H, Zhou S, Cao L (2015) Engineering the robustness of Saccharomyces cerevisiae by introducing bifunctional glutathione synthase gene

Store at 20C in a dry, light-protected environment

At predesigned time points, 25-l aliquots were collected, subjected to three freezethaw cycles (liquid nitrogen and 37 C) and transferred to a 96-well plate (25 l per well)

Besides, biochemical, pathological, and immunohistochemical assessments were performed to evaluate if CCM could afford therapeutic effectiveness against GEN or NaS accompanied ototoxic effect