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2020 Oct 1;21(19):7278

(2020) showed that RPE isolated from AMD donor samples had downregulated AMPK/SIRT1 activity and decreased NAD+ which had an impact on reduced PGC-1 levels by increased acetylation and (inactivation of PGC-1), a master regulator for mitochondrial biogenesis when compared to age-matched control samples

On incubation at room temperature, the solution was reacted with assay mixture (10 mM sulphanilamide with 7 mM -napthyl amine) and kept under dark for 1 h