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The supernatant was discarded carefully and the cells were resuspended in 3 ml of chilled (80 mM CaCl 2 and 50 mM MnCl 2 ) buffer and kept on ice for 30 min

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Besides, EGFR is a protein tyrosine kinase expressed on many types of tumor cells, including breast, ovarian, bladder, head and neck, and prostatic carcinoma