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Positive amplicons (visualization of a 300 bp 16S band on the agarose gel) were normalized using the SequalPrep normalization kit (Thermo Fisher Scientific A1051001), and resultant PCR products were sequenced by the McMaster Genomics Facility (Hamilton, ON), using the Illumina MiSeq platform

[9] Tras una serie de cuidadosos estudios clnicos, George Richards Minot y William Parry Murphy, se propusieron aislar parcialmente la sustancia presente en el hgado causante de la cura de la anemia en los perros, y descubrieron que era el hierro

Feng, H