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In addition to increased mRNA expression, we observed G6PD induction at the protein level under both acidosis and lactic acidosis (Figure 4F) and both MCF-7 and ZR-75-1 cells had increased intracellular G6PD activity under acidosis (Additional file6: Figure S5C)

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Discussion Plasma TMAO reached a level that was comparable with the previously reported level following 12 weeks of supplementation in older women [12], despite the administration of a lower LC dose