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16, e9596 (2020), Abstract Transcriptomics, metabolomics and lipidomics of chronically injured alveolar epithelial cells reveals similar features of IPF lung epithelium: Roque, W., Cuevas-Mora, K., et al

In brief, HEPES-buffered saline containing 0.5 mm EGTA (ethylene glycol-bis(-aminoethyl ether)-N,N,N,N-tetraacetic acid)and then a digestion medium with collagenase (100 digestive units/ml) were perfused via the inferior vena cava, and isolated cells were plated on collagen I-coated coverslips in DMEM supplemented with 10% FBS