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Immunogenicity: Some carrier polymers, e.g., poly-lactic acid and poly-lactic-co-glycolic acid, may activate unexpected immunological responses and generate inflammatory byproducts, which can impair therapeutic efficacy and health safety ( Reproducibility: Reproducibility challenges arise during large-scale production of protein-based transporters because variations in protein properties (e.g., molecular weight, purity, stability, etc.) can negatively affect the functional properties and delivery performances of transporters