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39 Besides causing tissue injury, ROS can also activate the NF-kB pathway to amplify inflammation through upregulation of expression of multiple genes, such as IL-6, TNF-, and chemokines

The results demonstrate that this combination therapy is more effective than individual treatments in reducing oxidative stress and enhancing antioxidant and autophagic pathways via the p62/Keap1/Nrf2 signaling mechanism (Aqeel et al

RRID:Addgene_72876)) for 48 h before seeding for flux analysis at 10,000 cells per well and allowed to adhere overnight in full culture media under normal conditions