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Consistent with this, patient-derived xenograft (PDX) models with genetic depletion or enzymatic degradation of GAGs exhibited reduced LDL uptake and impaired tumor growth, reinforcing GAG biosynthesis as a critical determinant of tumor growth by mitigating lipid oxidative stress

After primary antibody incubation, slices were then washed with PBS three times and incubated with secondary antibody donkey anti-mouse IgG (H + L) Alexa Fluor 488 (1:1000, Thermofisher Scientific, A-21202) in blocking solution for 90 min

Moreover, a clear association between lactylation modification and both heart failure and atherosclerosis has been established

Hyperkalemia and Hypokalemia in CKD: Prevalence, Risk Factors, and Clinical Outcomes