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Moreover, the introduction of novel molecular componentssuch as fusion proteins or transplanted mitochondriaposes immunogenicity risks, as these elements may be recognized as foreign antigens and elicit host immune rejection

J Phys Chem B 126:36593672

Kumar S, Stecher G, Li M, Knyaz C, Tamura K

From Day 0 to Day 2, the medium was replaced daily with N2 medium consisting of DMEM-F12/Glutamax (Thermo Fisher Scientific 10565042), N2 supplement (Thermo Fisher Scientific 17502048), BDNF (20 ng/ml, Peprotech 450-02), Lam (1 g/ml, Thermo Fisher Scientific 23017-015), MEM-NEAA (1X, Thermo Fisher Scientific 11140050), and doxycycline (4 M, STEMCELL Technologies 72742)