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IGF-1 LR3 is used in vitro preclinical research investigating insulin-like growth factor receptor (IGF-1R) signalling pathways

Lung-on-chip also revealed to be an ideal platform for infectious diseases study, where the complex and dynamic device microenvironment generates essential data not achievable with classic 2D in vitro models (130, 131)

For 96-well plates, 10 L of prepared reagent (1:50 ratio of monochlorobimane (MCB) to Tris assay buffer, per manufacturers instructions) was added to wells containing 100 L of culture media and incubated for 30 min at 37 C, washed with PBS, and imaged using an Olympus IX70 microscope with a DAPI filter (Excitation/Emission: 358 nm/461 nm)

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