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5.2 Analytical Platforms and Quality Control for GSH/GSSG Measurement Once sample integrity has been protected, the analytical platform determines selectivity, sensitivity, throughput, and whether GSH and GSSG are measured directly

[DOI] [PubMed] [Google Scholar] 84.Dolcourt B.A., Paxton J.H., Bora K.M., Aaron C.K

Usually more than one treatment will be recommended

The experiments with isolated mitochondria (0.5 mg/mL) were carried out at 28C with a standard reaction medium containing 15 M Ca 2+ , 10 mM Hepes, 125 mM sucrose, 2 mM K 2 HPO 4 , 65 mM KCl, 1 mM MgCl 2 , and 5 mM glutamate plus 5 mM malate (pH 7.2)