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doi: 10.1210/jc.2018-02414 65 ZhangXYeungDCYKarpisekMStejskalDZhouZ-GLiuFet al

Aggressive shaking can shear these chains, destroying the compound and rendering your research useless

Incubation of a pancreatic section with rabbit anti-glucagon (also of rabbit origin as for GPX1 antibody employed in this study) in combination with guinea pig anti-insulin and mouse anti-CD45 (antibody of mouse origin as for glucagon) showed the expected staining of alpha cells, beta cells and CD45-positive cells, respectively (ESM Fig