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Age-related changes in expression of tissue inhibitor of metalloproteinases-3 associated with transition from the notochordal nucleus pulposus to the fibrocartilaginous nucleus pulposus in rabbit intervertebral disc

Moreover, it has been previously shown that GPX4 overexpression in cancer cells inhibits RSL3-mediated ferroptosis, whereas GPX4 deletion increases susceptibility to ferroptosis [22]

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Then, the cells were cultured in a T-75 tissue culture flasks after filtered through a 70 m nylon cell strainer