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Description

RNA was reverse-transcribed in a 40 l reaction using M-MLV reverse transcriptase and random hexamers, according to the manufacturers protocol (Life Technologies)

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For research-supply positioning, this question should be reframed around experimental protocol design rather than use instructions

The untreated and MSO-treated parasite pellets isolated by saponin treatment were pooled separately during solubilization with 6 M urea in 25 mM ammonium bicarbonate buffer