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After sonicating (1400 rpm, 20 C, 5 min), the extracts were cleared by centrifugation (17,000 g , 20 C, 10 min), and the lipids in the supernatant were analyzed by LC-MS

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Pseudotemporal analysis of Cdkn1a, H2ax, and Il6 revealed that, compared to naturally aging rats, HHcy accelerated the upregulation of these senescence-associated genes (Figure 2I)

These systems enhance bioavailability through solubility modulation, supersaturation generation via spring and parachute effects, simultaneous improvements in solubility and permeability, and protection of chemical stability (236)