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Results A counter-selection system was engineered in Saccharomyces cerevisiae based on the known feedback inhibition of gamma-glutamylcysteine synthetase ( GSH1 ) gene expression, which is rate limiting for GSH synthesis: the GSH1 ORF and the counter-selectable marker GAP1 were expressed under control of the TEF1 and GSH-regulated GSH1 promoters, respectively

Polysorbate 60 is sorbitol-based and created from the ethoxylation of sorbitan

The body's GH axis evolved to operate in pulses because continuous GH receptor stimulation leads to receptor downregulation and desensitisation

The formula of Livak and Schmittgen [21] was used for quantification of samples and normalization was per- formed using the housekeeping gene YWHAZ