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Description

IgG N-glycosylation from patients with pemphigus treated with rituximab

10.3892/or.2019.7189 Oncol

In vitro Methyltransferase assays The DNA sequences corresponding to mouse Mettl9 -FLAG and Stmn1 -HA were PCR amplified by a Phusion Plus DNA Polymerase (Thermo Fisher, #F630S) from pyCAG-METTL9-FLAG and pyCAG-STMN1-HA plasmids respectively (pyCAG plasmids generated in this study, as explained in other sections) with PCR primers (Supplementary Data File 4) including EcoRI and XhoI restriction sites

Increased expression of glutamine transporter Snat2/Slc38a2 promotes glutamine dependence and oxidative stress resistance, and is associated with worse prognosis in triple-negative breast cancer