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The overall biosynthetic pathway of isoprostanes involves the formation of arachidonyl radicals and peroxyl radical isomers with endocyclization, forming bi-cycloendoperoxide regiomers reduced to F2-isoprostanes (F2-IsoPs)

Advanced omics technologiesincluding high-throughput genomics, proteomics, lipidomics, and metabolomicsare anticipated to enhance our understanding of the composition and functions of PDEVs, particularly by resolving vesicle heterogeneity and identifying bioactive cargo signatures