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Emphasis is placed on the fundamental translational challenges revealed by GFLs-based clinical trials (such as GDNF and neurturin [NRTN]), including delivery bottlenecks, treatment timing, and limitations of disease models

The drug is then incorporated into the hydrogel, and the gel is injected or implanted into the body

Nonetheless, it remains a destructive technique that cannot monitor cellular kinetics in situ and can only provide a snapshot of the metabolic state at the time of analysis, which may not accurately reflect the dynamic nature of cells and their rapidly changing metabolic state (Wang et al., 2016)

Chen Y, Yang Q, Xu J, Tang L, Zhang Y, Du F, et al