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Protocol Overview At 10 mg/mL total a single 10 mg combo vial (5 mg + 5 mg) holds roughly 1 mL of usable solution

Proc Natl Acad Sci USA 2010;107:2687-92

135 VejchapipatP.TangkijvanichP.TheamboonlersA.ChongsrisawatV.ChittmittrapapS.PoovorawanY

Finally, the above approach to evaluate organoid-derived FVII function suggests that although these experiments are not specific for FVII activity per se, supernatants (supplemented with vitamin K) show hemostatic potential due to the secretion of serine proteases (probably gamma-carboxylated) into the conditioned medium that mimic the FVII effect