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Despite achieving complete remission following a combination of subtractive surgery and first-line chemotherapy, a substantial proportion of patients (range, 70-80%) experience recurrence within 2-5 years

Plasmids and transfection To construct 6xHis tagged FAT1, the FAT1 cadherin #1-9 (1-1139 aa)/cadherin #10-18 (1140-2081 aa)/cadherin #19-27 (2082-3023 aa)/cadherin #28-33 (3024-3789 aa) were cloned in pET28a vector to express recombinant FAT1 proteins in Escherichia coli strain BL21 (DE3)

Epileptogenesis is defined as the latent period between the initial brain insult and the first unprovoked seizure, where very intensive neuronal alterations take place, including oxidative stress, the expression of pro-inflammatory mediators, neurodegeneration, reactive gliosis, damage to the blood-brain barrier, increased epileptiform spiking, and spontaneous recurrent seizures (Borowicz-Reutt and Czuczwar 2020)

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